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mild hypotonic lysis buffer  (TaKaRa)


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    Structured Review

    TaKaRa mild hypotonic lysis buffer
    Mild Hypotonic Lysis Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hypotonic+buffer/Lysis+Buffer/pm41839890-367-27-39
    Average 97 stars, based on 3125 article reviews
    mild hypotonic lysis buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: The nucleolus is the site for inflammatory RNA decay during infection
    Article Snippet: .. LPS-stimulated RAW 264.7 cells were washed with cold PBS twice and then fractionated by hypotonic buffer containing PIC and recombinant RNase inhibitor (TaKaRa). ..

    Single Cell:

    Article Title: scSLAM-seq reveals core features of transcription dynamics in single cells
    Article Snippet: .. Single cells were immediately sorted using a FACSaria III (BD Biosciences; precision: single-cell; nozzle: 100 μm) into individual wells of a 48-well plate (Brand) filled with 4 μl of a hypotonic lysis buffer containing 2 U/μL RNase inhibitor (Takara) and 0.2 % Triton-X-100 in Nuclease-free water. ..

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Lysis:

    Article Title: scSLAM-seq reveals core features of transcription dynamics in single cells
    Article Snippet: .. Single cells were immediately sorted using a FACSaria III (BD Biosciences; precision: single-cell; nozzle: 100 μm) into individual wells of a 48-well plate (Brand) filled with 4 μl of a hypotonic lysis buffer containing 2 U/μL RNase inhibitor (Takara) and 0.2 % Triton-X-100 in Nuclease-free water. ..

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Article Title: DRP1 induces neuroinflammation via transcriptional regulation of NF-ĸB.
    Article Snippet: .. Reverse transcription and cDNA preamplification using Smart-seq2 method Laser microdissected cells (40 cells per cell type) were collected into 0.2-mL thin-wall PCR tubes containing 2 μL of mild hypotonic lysis buffer (0.2% Triton X-100 and 2 U/μL RNase inhibitor; Clontech). ..

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Article Title: DRP1 induces neuroinflammation via transcriptional regulation of NF-ĸB
    Article Snippet: .. Laser microdissected cells (40 cells per cell type) were collected into a 0.2 ml thin-wall PCR tube containing 2 μl of a mild hypotonic lysis buffer composed of 0.2% Triton X-100 and 2 U/μl of RNase inhibitor (Clontech). ..

    Polymerase Chain Reaction:

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Article Title: DRP1 induces neuroinflammation via transcriptional regulation of NF-ĸB.
    Article Snippet: .. Reverse transcription and cDNA preamplification using Smart-seq2 method Laser microdissected cells (40 cells per cell type) were collected into 0.2-mL thin-wall PCR tubes containing 2 μL of mild hypotonic lysis buffer (0.2% Triton X-100 and 2 U/μL RNase inhibitor; Clontech). ..

    Article Title: ROS Dynamics Delineate Functional States of Hippocampal Neural Stem Cells and Link to Their Activity-Dependent Exit from Quiescence
    Article Snippet: .. 400 GFP positive cells from each ROS class were sorted into a PCR tube containing 8.5 μL of a hypotonic reaction buffer and RNA was prepared using the SMARTer Ultra Low RNA HV Kit (Takara Bio) according to the manufacturer’s protocol. .. For both experiments, cDNA of polyadenylated mRNA was synthesized from RNA of the lysed cells using SmartScribe reverse transcriptase, a universally tailed poly-dT primer and a template switching oligonucleotide (Takara Bio).

    Article Title: Redox potential defines functional states of adult hippocampal stem cells
    Article Snippet: .. 400 GFP positive cells from each ROS class were FACsorted into a PCR tube containing 8.5 μ l of a hypotonic reaction buffer and RNA was prepared using the SMARTer Ultra Low RNA HV Kit (Takara Bio) according to the manufacturer’s protocol. .. For both experiments, cDNA of polyadenylated mRNA was synthesized from RNA of the lysed cells using SmartScribe reverse transcriptase, a universally tailed poly-dT primer and a template switching oligonucleotide (Takara Bio).

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Article Title: DRP1 induces neuroinflammation via transcriptional regulation of NF-ĸB
    Article Snippet: .. Laser microdissected cells (40 cells per cell type) were collected into a 0.2 ml thin-wall PCR tube containing 2 μl of a mild hypotonic lysis buffer composed of 0.2% Triton X-100 and 2 U/μl of RNase inhibitor (Clontech). ..

    Stripping Membranes:

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Article Title: Loss of CSL Unlocks a Hypoxic Response and Enhanced Tumor Growth Potential in Breast Cancer Cells
    Article Snippet: .. The picked single cell was released into RNase-free PCR strip tube containing hypotonic Smart-seq RNA lysis buffer consisting of 2.3 ml of 19:1 ratio of 0.4% TritonX-100 and RNase inhibitor (Clontech), 1 ml of 10 mM dNTP (Fermentas), 1 ml of 10 mM oligo-dT primer, and ERCC spike-in (Ambion) at 72oC for three min. 2. ..

    Reverse Transcription:

    Article Title: DRP1 induces neuroinflammation via transcriptional regulation of NF-ĸB.
    Article Snippet: .. Reverse transcription and cDNA preamplification using Smart-seq2 method Laser microdissected cells (40 cells per cell type) were collected into 0.2-mL thin-wall PCR tubes containing 2 μL of mild hypotonic lysis buffer (0.2% Triton X-100 and 2 U/μL RNase inhibitor; Clontech). ..



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